Notes:
- Results will be displayed in the
Results Table
tab.
- Calculation usually takes < 2 minutes.
- We use
MAST
for differential expression testing, implemented in Seurat's
findMarker()
function.
- Genes not detected in at least 25% of
either of the two groups are removed.- Genes showing less than 25% difference in the fraction
of detection between the two groups are excluded.Results legend:avg_logFC: log fold-chage of the average expression
between the two groups. Positive values indicate that
the gene is more highly expressed in the first group.pct.1: The percentage of cells where the gene is detected in the first group.pct.2: The percentage of cells where the gene is detected in the second group.p_val_adj: Adjusted p-value, based on bonferroni correction using all genes in the dataset.
Source:
Seurat's documentation