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To characterise the heterogeneity of RUNX1+ and RUNX1- fractions of the mouse prostate, we performed droplet-based scRNA-seq on the 10x genomics platform. We dissected individual lobes of intact/hormone naive and regressed/castrated mouse prostates, isolated from P2-Runx1:RFP reporter mice, expressing RFP as a surrogate of Runx1 expression. In order to compensate for the relative sparsity of RFP+ and RFP- cells respectively pre- and post-castration, we enriched for under-represented populations by FACS prior cell encapsulation. Individually sorted populations from each lobe were multiplexed using MULTI-seq lipid-tagged indices in order to minimize technical confounders such as multiplets and batch effects (McGinnis et al., 2019)
Mevel R., Steiner I., Mason S., Galbraith L., Patel R., Fadlullah MZH., Ahmad I., Leung HY, Oliveira P., Blyth K., Baena E., Lacaud G., (2020)
RUNX1 marks a luminal castration resistant lineage established at the onset of prostate development. bioRxiv 2020.06.19.161604; doi: https://doi.org/10.1101/2020.06.19.161604.
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